ltravioletSpectrometryof Serum Triglyceridesby a TotallyEnzymicMethodAdaptedto a CentrifugalAnalyzer

We describe a mlcromethod for determining serum tiI glycerides (triacylglycerols) with the centrifugal analyzer. Thistechniqueis based on the procedure of Bublitz and Kennedy [J. Blo!. Chem. 211, 951 (1954)J. The enzymic hydrolysis requires 10 mm at 30 #{176}C. Twenty-six serum triglyceride assays can be done in about 30 mm. Concentration and absorbance are linearly related up to 5.0 mmol/llter, but higher concentrations can be assayed by changing conditions slightly. Day-to-day reproducibility for the method was satisfactory (CV, 2.7 to 8.4%). Comparison of this method and two other methods for triglycerkies, the automated Hantzsch condensation method and a commercial enzymic method (Calbiochem), gave correlation coefficients of 0.976 and 0.990, respectively. Results are unaffected by the presence of endogenous serum ADP, pyruvic acid, phosphatases, or ATPase.